I have the same problem and it's very annoying because there are some file that can't be read in virtual server. I've uploaded the logs and you can see clearly that the numbers of sectors from an iso image is different from the image written with dvd-rw. Also isobuster reports different size.
image from dvd
2,295,104 blocks
4,700,372,992 bytes
iso image
2,183,040 blocks
4,470,865,920 bytes
And another problem is that imgburn doesn't remember the number of sectors to load from bootable disc tab after I reopen it.
dvdrw.log
iso.log