The Queue feature is designed to burn disc images, so it wouldn't work for your needs.
I'd do it this way: use advanced input build mode to add your files/folders until you reach the size of a DVD-R/DVD+R disc, then burn the disc right away or save the build project (for burning later), then repeat the process for the remaining files until you've burned all of them.
It'll be some 20+ single layer DVDs, so use high quality media such as Verbatim or Taiyo Yuden blanks and leave the Verify option enabled, or your backup will be as good as nothing.